Coding

Part:BBa_K3807001:Design

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


enCas9-PolI3M


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5899
    Illegal PstI site found at 5926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5899
    Illegal PstI site found at 5926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5899
    Illegal BamHI site found at 4168
    Illegal BamHI site found at 6186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5899
    Illegal PstI site found at 5926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5899
    Illegal PstI site found at 5926
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 5079
    Illegal AgeI site found at 4576
    Illegal AgeI site found at 6166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1159


Design Notes

Adapted from the original EvolvR paper (Halperin et al, 2018) by deleting theoredoxin binding domain. Deletion was made by PCR ampification around the TBD.

Source

Modified from part amplified from Addgene plasmid #113077

References

Halperin et al, 2018